Ion Channel Technology
ICR 8000 Ion Channel Reader
Invaluable for ion channel screening, the ICR 8000 - ion channel technology, detects ion channel activity using atomic absorption spectroscopy and
flux assays. Current applications include the evaluation of the hERG potassium
channel more quickly, more precisely and more accurately than other medium to
high throughput ion channel screening systems. Interactions with the hERG channel can lead
to prolonged QT syndrome. Thus, regulatory agencies require the assessment of
QT liability.
The
ICR 8000 displays broad utility; it can be applied to assess both voltage-gated
(hERG, BK/SK, Kv1.1, 1.4, 1.5, KCNQ, 2P,etc.) and ligand-gated
(KATP, nAChR, etc.) ion channels. This allows researchers to accelerate
drug development efforts in the treatment and prevention of diseases associated
with ion channels.
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Benefits |
Programmable and automated
• Automatic dilution, calibration
and cleaning
• Adaptable
to existing robotic automation
Sample
dilution not required
• High sensitivity
• On-line
dilution |
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Feautures |
•
Medium Throughput: Up to 5000 wells/day
• Single Channel: Measures 1 sample at a time
• Minimum sample volume: 50 µl
• Accommodation: 96/384-well microplates
• Footprint: H.67 cm X W.55 cm X D.37 cm
• Fuel Source: Acetylene / Compressed Air
• Options: Plate Stacker / Bar Code Reader
• Sensitivity: 0.05 ppm detection limit
• Precision: < 5% CV |
Eliminates
• Quenching effects associated with fluorescence
• Health
hazards associated with radioisotopes
• Radioisotope disposal
• Work
restrictions posed by Rb's
short half-life
Ideal
for hERG channel assays |
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ICR 12000 Ion Channel Reader
The ICR 12000 was designed for ultra
high throughput ion channel screening of compound libraries against ion channel targets. Based
on the robustness of Aurora Biomed's ICR technology, the reader offers high sensitivity
generating accurate drug rank order matching electrophysiology data. This new
member of the ICR ion channel screening series provides the throughput to access compound QT liability
early in the drug discovery process.
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Technology |
| The
technology is based upon the versatility, precision, and sensitivity of atomic
absorption spectroscopy (AAS) and Aurora Biomed's patented microsampling process.
Ion Channel Technology
is suitable for both ligand-gated and voltage-gated ion channels using cell-based
flux assays. |
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Feautures |
• High
Throughput: Up to 60,000 wells/day
• Multi-Channel: Measures 12 samples at a time
• Minimum sample volume: 20 µl
• Accommodation: 96/384-well microplates
• Footprint: H.135 cm X W.134cm X D.97cm
• Fuel Source: Natural Gas / Compressed Air
• Includes: Plate Stacker / Bar Code Reader
• Sensitivity: 0.05 ppm detection limit
• Precision: < 5% CV |
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Applications |
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ICR technology is suitable for many types of ion channels
broad applications
include:
• Shaker
(Kv1.1, Kv1.4 and Kv1.5)
• hERG
• KCNQ4
• B/SKCa
• KCNB/TASK-3
• Kir2.1
• nAChR
• KATP (P2X family) |
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