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HTS Flux Assay Automation

HTS Flux Assay Automation

Development & Validation of an Automated Workstation for High-Throughput Screening FLUX Assays

Automated liquid handling systems have been invaluable tools for carrying out high-throughput assays in the drug discovery and development field. Aurora Biomed validated the VERSA 1100 Workstation for automation of the cold-flux assay, a cell-based functional assay used for high-throughput screening of ion channel drug targets. The cold-flux assay protocol includes multiple reagent addition steps intermixed with multiple wash steps. All liquid handling steps were carried out by the VERSA Mini (110) and performance was comparable to manual performance (Z’ factor, window of detection and IC50 of standard blockers).