Manual library preparation is not scalable and is prone to human error. Automation of library preparation is much needed to not only reduce these issues, but to allow for the laboratory to increase overall sample throughput.
Targeted Next Generation Sequencing (NGS) technology is rapidly being adopted to assess the mutational status of multiple genes on formalin-fixed, paraffin-embedded (FFPE) tumor specimens in
clinical settings. Library preparation is a critical, hands-on and time-consuming step in the NGS workflow. During library preparation, each library is prepared in an independent well of a 96-well plate, encompassing several washes and magnetic bead-binding steps.
In this poster, which was presented at the 2015 American Molecular Pathology meeting, the laboratory of Catherine I. Dumur, PhD, Associate Director of Molecular Diagnostics Division and Professor of Pathology at Virginia Commonwealth University, presents the validation and implementation of the VERSA 1100 GENE for medium to high-throughput library preparation for routine utilization with the Ion AmpliSeq™ Cancer Hotspot Panel v2 (CHP2) assay on FFPE clinical specimens, including FFPE Quality Control (QC) materials.