Swift, Accurate and Efficient Solutions for Peptide Synthesis
SPOT synthesis technique utilizing cellulose supports, combined with Aurora’s liquid handling technology has given birth to the Versa series microarray spotter. Aurora’s extensive expertise in life science robotics has seen an increased need for scientific personnel to seek out automated solutions for peptide synthesis. The Versa series microarray spotter has been specifically developed for peptide synthesis, modules such as the nano-pipettor head make the simple distribution of reagents allowing you to conduct grams to milligrams of combinatorial chemistry using either contact or non-contact.
Spotted DNA Microarrays
The VERSA 1100 35-channel spotter is capable of printing cDNA microarray assays by an automated, robotic based, spot printing technique. The VERSA system accommodates a fixed pipetting pin setup, available in contact, or non-contact format, capable of dispensing nanolitre aliquots, down to 30nL.
- Spotting volume area – 300nl / spot, approximately 2mm in diameter
- Sample – nucleic acid
- Microarray surface – nylon membrane; 3mm between spot centres, 1mm spacing along perimeter
For DiagCor’s purpose, the assay is used to differentiate between diseases of the same genotype, on a nylon membrane microarray format.
The hybridization of the cDNA strands, labelled with a fluorescent dye, will illuminate with a lavender based colour signal
The non-specific bonding sequences can be easily washed with the ReagentDrop™, leaving the paired strands that have the strongest binding affinity.
The fluorescence signal that remains on each “spot” correlates to the strength of labelled target sequence that has effectively hybridized.
Quantification of this step can be conducted on downstream instrumental analyzers, which enable scanning of fluorescence signal, normalization, and data analysis.
Background & Application Areas
DNA microarray analysis identifies inflections in gene activity, that lead to small variations in DNA sequence, which are known as polymorphisms. Molecular analysis, targeted gene expression profiling, genetic disease detection, and non-invasive prenatal diagnosis are just some of the applications of DNA microarray analysis.
Further on that, SNP (single nucleotide polymorphisms) detection is a common and most frequent type of variation in the human genome that can be elucidated by DNA microarray analysis. Applications include forensic analysis, disease prognosis, genotyping, oncology based somatic mutation evaluations, and drug-target candidate identification.
The VERSA 110 SPOT printing workstation for peptide synthesis automates the process using Fmoc based chemistries. With an easy to use software and accommodation of low-cost porous membrane materials, it is an effective and robust pipetting system.
Specifically for peptide synthesis using the SPOT technique, the VERSA 110 is stable, and allows for high-throughput printing, higher-density microarrays, and allows for pipetting amino acids and reagents as low as 40nL.
The instrument is stable and robust, we have had zero breakdowns since it was installed many years ago. The VERSA SPOTTER would provide a more cost efficient solution, with higher density, higher accuracy and faster printing, all at a lower price. The minimum printing volume would be as low as 40nL.
– User Testimony.
Solid-Phase peptide synthesis involves linking of multiple amino acids via peptide bonds for the production of peptides. This process mimics the biological process of producing long peptides (proteins). In solid-phase peptide synthesis (SPPS), the peptide is ‘immobilized’ on a solid surface and can be retained during washing of liquid-phase reagents and synthesis by-products. Solid-phase peptide synthesis also allows the synthesis of natural peptides which are difficult to express in bacteria, the incorporation of unnatural amino acids, and peptide/protein backbone modification. In addition this process permits the synthesis of D-proteins generating extremely high yield in each step and provides a solution problems associated with sequence dependent synthesis.
At the National Taiwan University, the VERSA system is used to conduct experiments for cell microarrays, printed onto a microscope slide surface. The cell microarray is using as a tool to elucidate cell signaling pathway regulation and the impact of drug molecule binding interactions.
- Spotting volume area – 100nl – 300nl / spot, approximately 2mm in diameter
- Sample – cell solution (uniform, or different, printed onto 10×10 array format
- Microarray surface – 15x15mm microscope cover glass, immersed in culture medium
Once the cell solution is prepared onto a 10×10 array format, the corresponding drug molecules are printed onto the microscope surface onto cell clusters. After cultured for a period of time, the slide can be observed under a microscope to examine cell morphology.
- High-Throughput Reagent Kit Spotting
- Drug-Eluting Microarray
- Peptide Synthesis
- Tissue Microarracy
- CHIP Production
- Cancer Research
- Precision Medicine
- DNA Microarray
- Protein Microarray
- Biomedical Imaging