RNA Isolation Systems

AB96 Filter Column Binding RNA Kit

Isolation of total RNA from cultured cells and tissues in 96-well format

The AB96 Filter Column Binding RNA kit is designed to carry out the isolation of total RNA from cells and soft tissues using a standardized 96-well plate format. Up to 1x106 cells and 10mg soft tissues can be processed in each well of the 96-well RNA plate. Purified RNA can then be used for a variety of downstream applications such as RT-PCR, Northern Blotting, Nuclease Protection Assay and in vitro translation.

AB96 Magnetic Binding RNA Kit

Isolation of total RNA using magnetic beads for cultured cells

The AB96 Magnetic Binding RNA kit provides an efficient mechanism with which to carry out total RNA isolation. This kit allows for the rapid and reliable isolation of high-quality total cellular RNA from cultured cells. The kit combines the nucleic acid binding properties of our paramagnetic particles with an RNA Isolation system, thereby providing superior quality RNA. Unlike column based systems, the binding of nucleic acids to magnetic particles occurs in solution, which improves both binding kinetics and binding efficiency. Particles are completely re-suspended during the purification protocol by the subsequent wash steps, which enhances contact between the wash buffer and sample and removal of contaminants. This increases nucleic acid purity. The kit includes the Dnase I digestion step in order to remove genomic DNA contamination. The AB96 Magnetic Binding RNA Kit Dnase procedure can be fully automated using Aurora’s VERSATM series workstations.

AB96 Magnetic Binding FFPE RNA Kit

Isolation of RNA from the formalin-fixed, paraffin-embedded samples using magnetic particles

The AB96 Magnetic Binding FFPE RNA Kit is specially designed for isolation of total RNA from formalin-fixed, paraffin-embedded tissue sections. The sample is first processed in special buffer by heating to deactivated RNase/DNase and deparaffinization. Then the lysate is treated with Proteinase K to digest proteins. The cleared lysate is then mixed with magnetic binding particles to capture the RNA. After a few quick wash steps, purified RNA can be eluted with water or low salt buffer. It is a fast, short, and streamlined protocol that can be fully-automated. This kit ensures high quality RNA isolate through complete removal of contaminants and inhibitors.

For more information, please review Aurora’s High-Throughput Screening Kits brochure or contact us directly to discuss your specific application. Thank you for considering AURORA to solve your lab automation requirements.