Using non-radioactive assay as a screening tool for membrane protein modulators is well-documented in scientific literature and has been widely used for studying the potassium channel family. The same principle of the nonradioactive Rb+ assay can be easily applied to more membrane protein targets than currently validated. The following publications represent a partial collection of ICR applications useful to both pharmaceutical labs and academic institutions. Please contact Aurora for further information regarding your specific protocol requirements.

Flux Assay Overview

Nonradioactive Rubidium Efflux Assay Technology for Screening of Ion Channels

Georg C. Terstappen

Textbook cover for Ion Channel Screening Assay - Chapter 8

Ion Flux and Ligand Binding Assays for Analysis of Ion Channels

Georg C. Terstappen

Chapter 7 of Expression and Analysis of Recombinant Ion Channels

Technical Notes

Validation of endogenously expressed Na, K, -ATPase in Cor.At Cardiomyocytes using ICR8000

Target-Specific Publications

test table

Target   Publication by
Large Conductance Ca2+- Activated K+ Channels Functional Analysis of Large Conductance Ca2+ -Activated K + Channels: Ion Flux Studies by Atomic Absorption Spectrometry Abbott Laboratories
Calcium-Activated Chloride Channel Development and validation of HTS assay for screening the calcium-activated chloride channel modulators in TMEM16A stably expressed CHO cells Hebei Medical University
Cation-Chloride Co-transporter Development of Rubidium Flux Assay & HTS Campaign for Modulators of a Cation-Chloride Co-transporter Roche
Na+,K+,-ATPase Development of an HTS Assay for Na+,K+,-ATPase Using Nonradioactive Rubidium Ion Uptake Aurora Biomed
hERG Evaluation of the Rubidium Efflux Assay for Preclinical Identification of hERG Blockade Pfizer
hERG Analogs of MK-499 are differentially affected by a mutation in the S6 domain of the hERG K+ channel Merck
hERG Characterization of a hERG Screen Using the IonWorks HT: Comparison to a hERG Rubidium Efflux Screen Schering-Plough Research Institute
KCNQ2/3 Validation of an Atomic Absorption Rubidium Ion Efflux Assay for KCNQ/M-Channels Using the Ion Channel Reader 8000 Wyeth Research
KCNQ2 A medium-throughput functional assay of KCNQ2 potassium channels using rubidium efflux and atomic absorption spectrometry AstraZeneca
Nav1.7 Cellular HTS Assays for Pharmacological Characterization of NaV 1.7 Modulators AstraZeneca
TRPV1 Novel Methodology to Identify TRPV1 Antagonists Independent of Capsaicin Activation AstraZeneca
Kir6.2 Characterization of polyhormonal insulin-producing cells derived in vitro from human embryonic stem cells University of British Columbia