Automated Next Generation Sequencing (NGS) Library Preparation

PRODUCT INFORMATION


    VERSA’s innovative modules are designed to tackle the challenging steps of Automated NGS Library Preparation (also known as NGS automation). The Magnetic Bead Vortex ensures homogeneous bead suspension and distribution. The combination of ReagentDrop bulk reagent dispensing module and 96-Tip Aspirator offers fast and efficient bead washing steps while saving tip costs. The VERSA automated Next Generation Sequencing (NGS) library preparation and sample preparation workstations are compatible with various commercial available kits, reagents, and labware. VERSA automated NGS library and sample preparation workstations are highly scalable for different throughputs and budget.

    Specifications

    VERSA automated NGS library prep

    VERSA 1100 Automated NGS Library Preparation System

    Deck Capacity15
    Pipetting Head

    (Single Channel Function, Disposable Tip)

    8 or 96 channel or 4 channel with independent Z-axis
    ReagentDrop8 channels
    Magnetic Block1
    Temperature Regulation Block2
    Reagent Cooling Block1
    Plate Shaker1
    Gripper1
    96-Tip AspiratorOptional
    Liquid Level SensingOptional
    HEPA/UV/LED EnclosureIncluded
    Width x Depth x Height (cm)98.5 x 75.2 x 108.2
    Weight (kg)200

    Modules Tailored for Automated NGS Library Preparation Protocols

    magnetic-bead-vortex

    Magnetic Bead Vortex

    ensures homogeneous bead suspension

    Magnetic Bead Vortex

    ensures homogeneous bead suspension

    Heater Shaker with PCR plate

    Shaker-Heater

    allows efficient sample processing

    ReagentBlockCooler

    Reagent Block Cooler

    preserves precious dNTPs, buffers and enzymes

    ReagentDrop

    ReagentDrop

    draws reagents from their reservoir directly

    Gripper for VERSA 1100

    Gripper

    moves sample plates between different deck positions

    Magnetic Bead Separator

    pulls down magnetic beads for NGS automation

    96 Tip Aspirator

    increases efficiency and speed while reducing tip costs

    • Open System – compatible with commercially available kits including Illumina, Thermo Fisher, etc…
    • HEPA/UV/LED Enclosure – keeps samples contamination-free
    • Scalable Solution – processes 1 to 96 samples in parallel depending on throughput demands
    • Unparalleled Customer Service – our engineering team is readily available to troubleshoot your protocols

     

    VERSA Automated NGS Library Preparation Applications

    • DNA/RNA Purification and Cleanup
    • DNA/RNA Fragment Size Selection
    • Enzymatic Reaction Setup
    • Library Normalization and Pooling
    • Single and Multiplex RT-PCR Setup
    • Sequencing Reaction Setup
    • Oligo-based Gene Synthesis Setup
    • Magnetic Bead Based Applications

    Frequently Asked Questions

    Our VERSA automated workstations are very flexible and open systems and are compatible with almost all the Next Generation Sequencing (NGS) library and sample preparation kits, reagents and labware in the market.

    Yes, the VERSA Automated Nucleic Acid Extraction and Purification workstation is equipped with a Heater-Cooler Plate and Heater-Shaker that can be used for incubating samples at temperatures from 2 – 92°C.

    All the steps from DNA/RNA purification and cleanup, DNA/RNA fragment size selection, enzymatic reaction setup, library construction, normalization and pooling, single and multiplex real-time PCR setup, sequencing reaction setup can be automated on VERSA. Transferring the plate to thermocycler for PCR reaction and centrifuging steps should be done manually.

    Yes, when manual intervention is required, a pause message can be defined to pop up on the monitor to notify the user about the next manual steps. VERSA Automated NGS library preparation system can also email the pause notifications to users who should take responsibility for the step.

    No, the ReagentDrop dispensing speed on VERSA automated NGS library and sample preparation workstation is always optimized for reagents with different consistency to avoid splashing.

    No, we have validated VERSA for automating different applications and have not observed any cross-contamination issues. VERSA automated NGS library preparation workstation utilizes disposable tips to eliminate cross-contamination, additionally the aspirating and dispensing speed is carefully adjusted to avoid splashing or carry-over contamination. VERSA is also equipped with HEPA filter and UV light to keep the environment contamination-free.

    VERSA is a very flexible and open system and is capable of preparing libraries / samples for any NGS instrument.

    Get more information on the VERSA Automated NGS Library & Sample Preparation Workstations: