SARS-CoV2/IAV/IBV One-Step PCR Kit

PRODUCT INFORMATION


    SARS-CoV2/IAV/IBV One-Step PCR Kit

    The 2019-nCoV/IAV/IBV Nucleic Acid Test Kit has been developed for the detection of SARS-CoV-2, Influenza A and Influenza B RNA from human nasopharyngeal swabs, oropharyngeal swabs, sputum, alveolar lavage fluid specimens.

    The kit is CE certified and intended for in vitro diagnostic use only.

    Principle

    The SARS CoV2/IAV/IBV PCR kit is a one-step real-time reverse transcription polymerase chain reaction (RT-PCR) diagnostic kit.  Used for the detection of SARS-CoV-2  as well as Influenza A and B.

    The multiplex assay uses fluorescent probes and primers to detect three specific regions within the SARS-CoV-2 nucleocapsid (N) gene and the ORF1ab gene. β-globulin is used an internal control to monitor quality of the specimen and extraction process. Positive and negative controls are included. RNA isolated from patient samples can be directly used in the assay to  be reverse transcribed and amplified quantitatively. The pseudoviral particles used as positive control  act as control for the extraction process as well as the RT-PCR.

    Kit Components

    Components Main Ingredients
    1SARS-CoV-2 reaction mixture, lyophilizedPrimers, probes, PCR reaction buffer, dNTPs, Enzyme, etc.
    2SARS-CoV-2 positive control, lyophilizedLyophilized pseudoviral particles including ORF1ab and N genes detecting target sequence
    3Negative controlUltrapure water

     

    Analytical Specificity

    This test kit can be used to detect  SARS-CoV2

    Influenza A virus (2009 H1N1, H1N1, H3N2, H5N1, H7N9) and Influenza B virus (Yamagata, Victoria)  specifically.

    FAM channel detects SARS-CoV-2, HEX channel detects Influenza A, Cy5 channel detects Influenza B,ROX channel detects Human    internal reference gene as quality control.

    Limit of Detection

    • SARS-CoV2 (2019-nCoV ) : 400 copies/mL.
    • Influenza A virus : 1.5 TCID50/mL.
    • Influenza B virus : 2.0 TCID50/mL.

    Kit Specifications

    Validation

     Clinical study (900 cases)

    SARS-CoV2/IAV/IBV One-Step PCR amplification plot

     

    Get more information on Aurora’s One-Step PCR Kits: