Atomic fluorescence spectroscopy (also known as atomic spectrofluorometry or atomic fluorimetry) is a technique used in biochemical, medical, and chemical applications that require very high sensitivity as well as precision and accuracy. An atomic fluorescence spectrometer is capable of measuring samples containing both hydride-forming elements and Mercury at a parts per trillion (ppt) level using the unique vapour hydride generator. The high sensitivity and reliability of Aurora’s series of Atomic Fluorescence Spectrometers is ideal for elemental analysis in a variety of research and industrial sectors.
Metallurgy · Geology and Mining · Petrochemical · Pharmaceutical Quality Control · Agriculture and Environmental Monitoring
Frequently Asked Questions
In atomic fluorescence spectroscopy, similar to the related technique of atomic absorption spectroscopy, a sample absorbs light at a particular wavelength to promote its electrons from its ground electronic state into an excited state. From this excited electronic state, the electron drops down to a lower electronic state, emitting a photon with a specific wavelength in the process. By measuring the intensity of the emitted light at particular frequencies, it is possible to determine the concentration of the element in question via atomic fluorescence spectrometry.
The LUMINA series of Atomic Fluorescence Spectrometers provide sub-trace detection and elemental analysis of hydride-forming elements. The unique vapor/hydride generator provides enhanced sensitivities and reduced interferences for detection of Mercury (Hg), Arsenic (As), Cadmium (Cd), Zinc (Zn), Bismuth (Bi), Selenium (Se), Tellurium (Te), Antimony (Sb), Tin (Sn), Germanium (Ge), and Lead (Pb).
Detection limit for the following elements is specified in table in below:
|Element||Detection Limit (μg/L)||RSD (%)|
|As, Se, Pb, Bi, Sb, Te, Sn||0.01||<1.0|
What type of atomizer and gas/liquid separator technology is used in the LUMINA Atomic Fluorescence Spectrometer?
The LUMINA AFS system uses a quartz tube furnace with an interference-reducing Ar-H2 diffusion flame. This flame is ignited automatically within the instrument. The LUMINA AFS system is also equipped with a two-stage gas/liquid separator and a filtered, integrated exhaust system that can efficiently decontaminate pollutants during sample preparation and delivery steps.
Yes, the LUMINA AFS system utilizes the Cold Vapor method for mercury detection. Mercury analysis is a critical component of food and water safety, agriculture, and environmental monitoring. Samples must be digested prior to analysis to ensure accurate analyte measurements.
Is Atomic Fluorescence Spectroscopy a recommended method for Mercury (Hg) detection in environmental samples?
Yes, the Cold Vapor method used by the LUMINA Atomic Fluorescence Spectrometer is recommended by the USFDA for detection of Mercury at ppb or sub-ppb levels.
Yes; for example, the LUMINA AFS system has a detection limit for mercury for 1ppt, which is comparable to literature reported detection limits for mercury with ICP-MS. Additionally, the plasma temperatures utilized in ICP-MS may be too hot for accurate mercury analysis, leading to premature mercury decomposition and thus unreliable results.
Yes, the LUMINA includes an optional XYZ autosampler for sample introduction that hosts a maximum of 192 standard cup/test tube sample racks. It is compatible with standard solvent extraction test tubes, ICP test tubes, and custom-made trays.
The LUMINA Atomic Fluorescence Spectrometers are equipped with specially-designed high intensity Hollow Cathode Lamps (HCL) with 2-channel independent power supplies, providing improved sensitivities and lower detection limits. It also features dual channel, simultaneous two-element analysis, using a computer controlled pulsed light sources.