Monkey pox is a zoonotic virus, causing symptoms similar to small pox, though less severe clinically. It belongs to orthopoxvirus genus of Poxviridae family. Two clades of Monkeypox are known: the West African and the Congo Basin (Central African). It can be transmitted from one person to other through close contact with lesions, body fluids, droplets and contaminated material such as linen. The incubation period for infection is usually 6-13 days, however it can vary from 5 to 21 days. As per WHO, possible symptoms may include headache, fever, swollen lymph nodes, weakness, muscle and body ache. The Monkeypox virus can be detected by real time polymerase chain reaction, transmission electron microscopy and immunohistochemistry.
The Monkeypox Virus Real Time PCR kit is used for the detection of Monkeypox virus in serum or lesion exudate samples by using real time PCR systems
Each kit contains:
- 1 vial DNA Extraction Buffer
- 1 vial MPV Reaction Mix
- 1 vial PCR Enzyme Mix
- 1 vial Molecular grade water
- 1 vial Internal control (IC)
- 1 vial MPV positive control (1X107 copies/ml)
Analysis sensitivity: 5X103 copies/ml
Monkeypox virus is an orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target both human and animals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids. The genome is not segmented and contains a single molecule of linear double stranded DNA, 185000 nucleotide long. The Monkeypox Virus real time PCR kit contains a specific ready-to-use system for the detection of the Monkeypox Virus through polymerase chain reaction (PCR) in real time PCR system. The detection of amplified Monkeypox virus DNA fragments is performed in fluorimeter channel FAM with the fluorescent quencher BHQ1.
It is based on the fluorogenic 5’ nuclease assay. During the PCR reaction, the DNA polymerase cleaves the probe at the 5’end and separates the reporter dye from the quencher dye only when the probe hybridizes to the target DNA. This cleavage results in the fluorescent signal generated by the cleaved dye, which is monitored real-time by the PCR detection system. The PCR cycle at which an increase in the fluorescence signal is detected initially is proportional to the amount of the specific PCR product. Monitoring the fluorescence intensities in real-time allows the detection of the accumulating product without having to re-open the reaction tube after the amplification.
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