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Protein/peptide Purification

VERSA-1100, Automated liquid handling System, with 15-positioned modular deck configuration with options such as magnetic bead vortex, shaker-heater and ReagentDrop, For 96 well plates and 384 well plates.Aurora’s automated mass spectrometry sample preparation workstations for both Solid Phase Extraction (SPE) and Liquid-Liquid Extraction (LLE).

Protein/peptide Purification


Solid Phase Extraction (SPE) is one of the most common methods for preparing samples to be analyzed by Mass spectrometry. Common workflow runs samples down through a syringe cartridge with absorbents (often silica or carbon based) several times and the size for these cartridges is mostly 1ml, 3ml or 6ml. Although this cartridge-based method works perfectly fine with samples in large volume, it can create recovery issues when dealing with small volume (roughly 1ml in total) since there will not be enough sample to be thoroughly eluted into collection vials. Research on metabolomics or peptides often investigates various human samples in high throughput and thus come in small volume. Using an ordinary cartridge in these applications not only introduces a lower recover rate but is also inefficient since these loaded syringes take space and only a few can be run at the same time.

Fortunately, filtration plate has been developed to serve this purpose and there are multiple types available in the current market, each suits for a corresponding elute (from peptides to metabolites etc.). They have the exact layout/dimension as a standard 96-well plate which support a high-throughput (96 samples at a time) but low-volume (less than 1ml) extraction. These plates are easy to use as well – one can either apply a positive pressure (for example: Thermo’s SOLAµ™ SPE Plates) or a vacuum manifold (for example Phenomenex’s Novum ™ SLE Plates) to push eluents through the filter and the whole workflow looks almost identical to the ones using ordinary carbon cartridges.

With the help of these filtration plates, research scientists in peptide or metabolomics can finally push SPE method to its limit and with the help of an automated liquid handler, it can even pass beyond the limit!

John Hopkins All Children Hospital has been using Novum SLE plates for their metabolomics research and with the assistance of VERSA 1100 SPE, to automatically prepare patient samples for GC/MS analysis on a monthly basis.  The VERSA 1100 SPE Workstation offers flexibility in sample throughput by accommodating 1, 3, and 6 mL cartridges, and 96-well SPE cartridge plate formats. With 15 deck positions and a variety of dedicated modules, including positive and negative pressure applicators, nitrogen dryer, and a shaker-heater, the system’s versatility can be extended to include sample extraction as well as derivatization and reconstitution for further analysis. Highly compatible with consumables from different brand in the market, Aurora is pleased to present VERSA with a fully automated solution to our valued clients!

Novum_plate

Specifications

VERSA 1100 Automated Solid Phase Extraction (SPE) System

Deck Capacity15
Pipetting Head

(Single Channel Function, Disposable Tip)

4 or 8 channel
ReagentDropup to 8
Supported Cartridges1, 3 or 6 mL cartridges or 96 SPE cartridge plate
GripperGripper
Pressure Module(s)4 channel positive pressure module and/or vacuum manifold (negative pressure)
Nitrogen Dryer24 or 96 channels
Shaker-Heater (2400rpm, RT to 90°C)Optional
Reagent Block CoolerOptional
Liquid Level SensingOptional for Liquid Liquid Extraction
HEPA/UV/LED EnclosureIncluded
Width x Depth x Height (cm)99 x 75 x 90
Weight (kg)162

 

Specifications

VERSA HT (2100) Automated Solid Phase Extraction (SPE) System

Deck Capacity24 Deck Positions
Sonication Station4 Deck Positions
Pipetting Head (Single Channel Function, Disposable Tip)4 Channel Pipette (1000uL)
ReagentDrop5 Channel Reagent Drop (bulk dispensing)
Supported CartridgesSPE cartridge compatible
Pressure Module(s)4 channel positive pressure module and/or vacuum manifold (negative pressure)
Nitrogen Dryer24 or 96 channels
Shaker-Heater (2400rpm, RT to 90°C)Optional / 4-in-1 Shaker for 4x Sample Tube Racks 4×6
Reagent Block CoolerOptional
GripperGripper
HEPA/UV/LED EnclosureIncluded
Width x Depth x Height (cm)120 x 84 x 200 (Including Bench)
Weight (Kg)~500

Applications – VERSA Automated Solid Phase Extraction Systems

  • Food Safety
  • Vitamin D from Blood/Serum Samples
  • Drugs of Abuse in Forensic Analysis
  • Compounds for Drug Discovery
  • Iso-Flavonoides from Urine Samples
  • Aflatoxins from Seafood Products
  • Growth Promoters in Blood
  • Semi-Volatile or Non-Volatile Analytes
  • Protein Precipitation
  • Environment Health
  • Metabolite Extraction

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