Protein/peptide Purification

Fortunately, filtration plate has been developed to serve this purpose and there are multiple types available in the current market, each suits for a corresponding elute (from peptides to metabolites etc.). They have the exact layout/dimension as a standard 96-well plate which support a high-throughput (96 samples at a time) but low-volume (less than 1ml) extraction. These plates are easy to use as well – one can either apply a positive pressure (for example: Thermo’s SOLAµ™ SPE Plates) or a vacuum manifold (for example Phenomenex’s Novum ™ SLE Plates) to push eluents through the filter and the whole workflow looks almost identical to the ones using ordinary carbon cartridges.

With the help of these filtration plates, research scientists in peptide or metabolomics can finally push SPE method to its limit and with the help of an automated liquid handler, it can even pass beyond the limit!

John Hopkins All Children Hospital has been using Novum SLE plates for their metabolomics research and with the assistance of VERSA 1100 SPE, to automatically prepare patient samples for GC/MS analysis on a monthly basis.  The VERSA 1100 SPE Workstation offers flexibility in sample throughput by accommodating 1, 3, and 6 mL cartridges, and 96-well SPE cartridge plate formats. With 15 deck positions and a variety of dedicated modules, including positive and negative pressure applicators, nitrogen dryer, and a shaker-heater, the system’s versatility can be extended to include sample extraction as well as derivatization and reconstitution for further analysis. Highly compatible with consumables from different brand in the market, Aurora is pleased to present VERSA with a fully automated solution to our valued clients!