Next generation sequencing (NGS) technologies are emerging as a powerful means for providing gigabases of genetic information. Increased throughput and reduced sequencing costs have revolutionized genomics research.
Several methods are available to assess vitamin D sufficiency through measurement of serum 25-hydroxy vitamin D [25(OH)D]. Antibody (ELISA) or other protein-binding assays are available, but may not accurately report total vitamin D values. Moreover, manual sample preparation methods for assays such as LC-MS/MS or HPLC are very cumbersome.