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Vitamin D Liquid Liquid Extraction using VERSA Workstation

VERSA 1100 automated Liquid-Liquid Extraction System for Vitamin D

Vitamin D deficiency is associated with many pathological conditions including diabetes, osteoarthritis, and many forms of cancer.

Several methods are available to assess vitamin D sufficiency through measurement of serum 25-hydroxy vitamin D [25(OH)D]. Antibody (ELISA) or other protein-binding assays are available, but may not accurately report total vitamin D values. Moreover, manual sample preparation methods for assays such as LC-MS/MS or HPLC are very cumbersome.

Aurora’s VERSA™ LLE Workstation has been validated for the organic solvent extraction of 25(OH)D from blood serum using liquid liquid extraction (partition chromatography). Reliable reproducibility of peak height data from the extractions suggests that biomolecules like 25(OH)D can be effectively extracted using this automation within acceptable values. Moreover, the automated process takes 60 minutes for a set of 96 samples with no detectable cross contamination. The extraction process took approximately 17 minutes less than the manual procedure.

25-hydroxy vitamin D extraction using the VERSA LLE Workstation is just one of many application solutions offered by Aurora. Numerous applications that are widely used have been automated, and each VERSA workstation remains customizable to accommodate unique needs. Aurora provides complete lab automation solutions supporting life science, chemical analysis and drug discovery research by supplying application support, reagents and instruments which offer higher throughput while improving quality, accuracy, and precision.