Automated solid-phase peptide synthesis involves linking of multiple amino acids via peptide bonds for the production of peptides. This process mimics the biological process of producing long peptides (proteins). In solid-phase peptide synthesis (SPPS), the peptide is ‘immobilized’ on a solid surface and can be retained during washing of liquid-phase reagents and synthesis by-products. Solid-phase peptide synthesis also allows the synthesis of natural peptides which are difficult to express in bacteria, the incorporation of unnatural amino acids, and peptide/protein backbone modification. In addition this process permits the synthesis of D-proteins generating extremely high yield in each step and provides a solution problems associated with sequence dependent synthesis.
Aurora’s VERSA Microarray Printer is a liquid handling platform for performing automated solid-phase peptide synthesis. It is capable of automatic pipetting for sub-microliter dispensing and contact array printing applications in contact printing applications. This system spots Fmoc or t-Boc amino acid residues in dimethylformamide (DMF) on to solid surfaces, such as membranes, slides, filter paper or any other suitable array surface, in user-defined sequences. This workstation automates solid phase peptide synthesis in a simple and reproducible manner with extremely high yield and accuracy. The robotic arm is controlled by an external computer, allowing the user to micro-control the desired range of movement, sequence of individual peptides and standardize the spacing between spots.