The polymerase chain reaction (PCR) is a highly sensitive technique, enabling researchers to rapidly select and amplify a specific target sequence from a background of non-related sequences. It has been demonstrated that it is possible to perform PCR on samples adhered to microscope slides. In situ on slide PCR enables effective detection of low-copy sequences and permits cell-by-cell gene expression testing.
Advalytix’s AmpliSpeed slide thermocycler was specifically designed as a compact thermocycler capable of performing all PCR reactions on a standard glass slide. AmpliGrid glass slides have a special chemically structured surface with DNA free reaction centers. 5 µL of sealing solution must be applied to each droplet on the slide surface. This platform enables users desiring ultra low volume tests in the 1 µL range. This novel system maintains superior convenience, speed, sensitivity, efficiency and reproducibility. It is possible to analyze according to a cell by cell and/or gene by gene approach. Any template material that may be fixed to slides – including clinical or forensic stains and purified nucleic acids can be used with this approach.